Phage auxiliary genes

Phages replicate in bacterial hosts by repurposing host cellular machinery towards the production of phage progeny. To exploit host cell resources, many phages encode auxiliary proteins that interfere with essential biological processes of the bacterial host. Identification and characterization of phage auxiliary proteins may yield tools for curtailing bacterial infections. Additionally, the discovery of phage-targeted host pathways could reveal potential antibacterial therapeutic targets. We are performing genome-wide temporal transcriptomic, proteomic and transposon profiling of phage infected Enterococcus faecalis to identify factors involved in phage bacterial interactions (Chatterjee et al. 2020). Mechanistic characterization of phage auxiliary factors that target important physiological processes, may aid in the development of novel therapeutic strategies for the control of enterococcal infections.