DUERKOP LAB

 

IMMUNOLOGY

&

MICROBIOLOGY

@ CU ANSCHUTZ

The Duerkop Lab would like to thank the following funding sources 

RESEARCH

The Duerkop lab studies bacterial viruses (bacteriophages or phages) and their interactions with their hosts. The long-term goal of the Duerkop lab is to understand how phages and other forms of mobile DNA contribute to host-microbe interactions in the intestine and their overall impact on human health.

Our lab seeks to: 1) Determine the mechanisms underlying phage interactions with multidrug resistant enterococci and how phages impact enterococcal colonization of the intesitne; 2) Understand how native CRISPR-Cas systems function as barriers to nucleic acid acquisition in the intestine and how CRISPR-Cas contributes to the genetic diversity and ecology of the microbiota; 3) Explore the role phage communities play in maintaining a healthy intestinal microbiota and assess whether intestinal phages can shape the composition of the microbiota leading to chronic intestinal inflammation. 

 
 

PROJECTS

PUBLICATIONS

2019

Chatterjee A, Willett JLE, Nguyen UT, Monogue B, Palmer KL, Dunny GM, Duerkop BA. Parallel genomics uncover novel enterococcal-bacteriophage interactions. bioRxiv. available at: https://doi.org/10.1101/858506 

 

Johnson CN and Duerkop BA. Dyeing to connect. Nat Microbiol. 2019;4(12):2033-2034. doi: 10.1038/s41564-019-0616-1. PMID: 31754272. View in PubMed  

Rodrigues M, McBride SW, Hullahalli K, Palmer KL  and Duerkop BA. Conjugative delivery of CRISPR-Cas9 for the selective depletion of antibiotic-resistant enterococci. Antimicrob Agents Chemother. 2019;63(11). doi: 10.1128/AAC.01454-19. PMID: 31527030. View in PubMed

Price VJ, McBride SW, Hullahalli K, Chatterjee A, Duerkop BA, Palmer KL. Enterococcus faecalis CRISPR-Cas is a robust barrier to conjugative antibiotic resistance dissemination in the murine intestine. mSphere. 2019;4(4). doi: 10.1128/mSphere.00464-19. PMID: 31341074. View in PubMed

 

Chatterjee A, Johnson CN, Luong P, Hullahalli K, McBride SW, Schubert AM, Palmer KL, Carlson PE Jr and Duerkop BA. Bacteriophage resistance alters antibiotic-mediated intestinal expansion of enterococci. Infect Immun. 2019;87(6). doi: 10.1128/IAI.00085-19. PMID: 30936157. View in PubMed

 

Chatterjee A and Duerkop BA. Sugar and fatty acids Ack-celerate prophage induction. Cell Host Microbe. 2019;25(2):175-176. doi: 10.1016/j.chom.2019.01.012. PMID: 30763529. View in PubMed

2018

Duerkop BA. Bacteriophages shift the focus of the mammalian microbiota. PLoS Pathog. 2018;14(10):e1007310. doi: 10.1371/journal.ppat.1007310. PMID: 30359456. View in PubMed

Duerkop BA, Kleiner M, Paez-Espino D, Zhu W, Bushnell B, Hassell B, Winter SE, Kyrpides NC and Hooper LV. Murine colitis reveals a disease-associated bacteriophage community. Nat Microbiol. 2018;3(9):1023-1031. doi: 10.1038/s41564-018-0210-y. PMID: 30038310. View in PubMed

Chatterjee A and Duerkop BA. Beyond Bacteria: Bacteriophage-eukaryotic host interactions reveal emerging paradigms of health and disease. Front Microbiol. 2018;9:1394. doi: 10.3389/fmicb.2018.01394. PMID: 29997604. View in PubMed   

 
 

PEOPLE

Breck Duerkop (Assistant Professor)

breck.duerkop@cuanschutz.edu

Breck received his BS in Biology from the University of Wisconsin-Milwaukee and a PhD in Microbiology from the University of Washington. He completed postdoctoral training at the University of Texas Southwestern Medical Center. Breck joined the faculty in the Department of Immunology and Microbiology at the University of Colorado School of Medicine in December of 2016. 

Anushila Chatterjee (Postdoctoral Fellow)

anushila.chatterjee@cuanschutz.edu

Anushila attended the University of Calcutta, India where she obtained both a BS and MS in Microbiology. She received a PhD in Biochemistry from the State University of New York at Buffalo. Her research focuses on E. faecalis-phage interactions and the study of phage auxiliary genes. 

Mike Mangalea (Postdoctoral Fellow)

mihnea.mangalea@cuanschutz.edu

Mike attended the University of North Carolina-Chapel Hill where he received a BA in Biology. He conducted his PhD work in Microbiology at Colorado State University. Mike's research combines culture based methods and metagenomics for the study intestinal viruses and aims to explore the utility of phages as biomarkers of intestinal disease.       

Greg Canfield (Infectious Disease Fellow)

gregory.canfield@cuanschutz.edu

Greg earned a BS in Biochemistry from Niagara University and completed MD, PhD training in Microbiology at the State University of New York at Buffalo. He completed an Internal Medicine Residency at Scripps Green Hospital/Scripps Clinic and is now an Infectious Disease Fellow at the University of Colorado School of Medicine. Greg is studying the molecular interactions between phages and multidrug resistant clinical isolates of E. faecium.  

Cydney Johnson (PhD Student)

cydney.johnson@cuanschutz.edu

Cydney attended Rhodes College where she earned a BS in Biology and a minor in Spanish. She joined the Biomedical Sciences Graduate Program at the University of Colorado School of Medicine in 2017. Cydney's research focuses on the biochemistry of enterococcal phage receptors and mechanisms of enterococcal transmission among mammalian hosts.     

Micah Keidan (Lab Manager)

micah.keidan@cuanschutz.edu

Micah received a BS in Genomics and Molecular Genetics from Michigan State University. Micah's projects relate to the study of phages for the decolonization of intestinal enterococci and CRISPR-cas as a barrier to horizontal gene transfer in the intestinal microbiota.    

Viviane Schink (Visiting Master's Student)

viviane.schink@rwth-aachen.de

Viviane is currently pursuing a Master's degree in Molecular and Applied Biotechnology at the RWTH Aachen University in Germany. She is completing an internship in the Duerkop Lab where she is studying phage-bacteria co-evolution during continuous culturing of E. faecalis with phages.     

Lab Alumni

Jason Hickman (Visiting Scientist, 2017)

Current position: Senior Scientist, Archer Daniels Midland (AMD), Decatur, IL.

 

Phat Luong (Master's Student, 2017-2018)

Current position: Data Science to Patient Value, Denver Veterans Affairs, Aurora, CO.

Sara McBride (Lab Manager, 2017-2019)

Current position: Laboratory Coordinator, Salk Institute, La Jolla, CA.

CONTACT

The Duerkop Lab is located at the University of Colorado School of Medicine's  Anschutz Medical Campus in Aurora, Colorado, 8 miles from downtown Denver and 20 miles from the foothills of the Rocky Mountains   

12800 E. 19th Ave. 

Research Complex I

P18-9402D

Aurora, CO, 80045

Tel: 303-724-0940

Fax: 303-724-4226